| 摘要 |
<p>A method for in-situ focus-fusion multi-layer spectral imaging (2) and analysis of depth dependent particulate samples. A unique method of focus-fusion is applied to focused and defocused images acquired from multi-layer spectral imaging (2) of a depth dependent particulate sample, in order to construct focused fused cube spectral image (5) representations of the imaged particles, thereby generating a focused image of essentially each particle in the sample. The method features the use of a uniquely defined and calculated focus-fusion factor parameter (5(c)) which combines empirically determined particle morphological and biological characteristics (5(b)) with empirically determined particle spectral characteristics, and is used in critical steps of image detection and image analysis classification (9). This uniquely determined parameter enables achievement of high levels of accuracy and precision in detection and classification of the sample, in general, and of the featured particles, in particular.</p> |
