Device and method for determination of an analyte in blood

摘要

A sample acquiring device, an apparatus and a method for spectrophotometric measurement of high density lipoprotein (HDL) and at least one of total cholesterol (TC), triglycerides (TG) and glucose (FPG).

主权项

1. A sample acquiring device comprising:
at least one inlet for receiving a body fluid sample, at least one capillary sample reception cavity in capillary communication with said at least one inlet, a first pathway in communication with said at least one inlet and a first measurement cavity, and a second pathway in communication with said at least one inlet and a second measurement cavity, the second measurement cavity being separate from said first measurement cavity, wherein the first pathway and the second pathway are in communication with said at least one capillary sample reception cavity such that spontaneous flow from the capillary sample reception cavity is prevented and such that body fluid sample is forcible from the capillary sample reception cavity into a first centrifugation cavity of said first pathway and a first centrifugation cavity of said second pathway by subjecting the sample acquiring device to a first centrifugation, wherein said first centrifugation cavity of said first pathway is in capillary communication with a first capillary cavity of said first pathway and said first centrifugation cavity of said second pathway is in capillary communication with a first capillary cavity of said second pathway, such that at least a fraction of said body fluid sample in said first centrifugation cavity is transferable from said first centrifugation cavities to said first capillary cavities, wherein, each of the first centrifugation cavities of the first pathway and of the second pathway has a lower portion and an upper portion oriented relative each other such that centrifugation of the sample acquiring device forces any sample in the first centrifugation cavity towards the lower portion, wherein the first capillary cavity of the first pathway is in capillary communication only with the upper portion of the first centrifugation cavity of the first pathway, wherein the first capillary cavity of the second pathway is in capillary communication only with the upper portion of the first centrifugation cavity of the second pathway, wherein a precipitation agent is provided in said first capillary cavity of said second pathway, wherein said first capillary cavity of said first pathway is in communication with a second centrifugation cavity of said first pathway and said first capillary cavity of said second pathway is in communication with a second centrifugation cavity of said second pathway, such that spontaneous flow from said first capillary cavities is prevented and such that said at least a fraction of said body fluid sample from the first capillary cavities is forcible from the said first capillary cavity of said first pathway and into a second centrifugation cavity of said first pathway and from said first capillary cavity of said second pathway to a second centrifugation cavity of said second pathway by subjecting the sample acquiring device to a second centrifugation, wherein said second centrifugation cavity of said first pathway is in capillary communication with a second capillary cavity of said first pathway and said second centrifugation cavity of said second pathway is in capillary communication with a second capillary cavity of said second pathway, such that at least a fraction of said body fluid sample in said second centrifugation cavities is transferable from said second centrifugation cavities to said second capillary cavities, wherein said second capillary cavity of said first pathway is in communication with a transfer cavity of said first pathway and said second capillary cavity of said second pathway is in communication with a transfer cavity of said second pathway, such that spontaneous flow from said second capillary cavities is prevented and such that at least a fraction of said body fluid sample in said second capillary cavities is forcible from said second capillary cavity of said first pathway into said first measurement cavity via said transfer cavity of said first pathway and from said second capillary cavity of said second pathway into said second measurement cavity via said transfer cavity of said second pathway by subjecting the sample acquiring device to a third centrifugation, wherein at least one chromophore is provided in said second capillary cavity, in said transfer cavity or in said first measurement cavity of said first pathway, and wherein at least one chromophore is provided in said second capillary cavity, in said transfer cavity or in said second measurement cavity of said second pathway.