| 摘要 |
<p>It is intended to provide a polypeptide usable in producing a cyclic tetrasaccharide having a cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→} structure, a DNA encoding this polypeptide and use of the same. This object is achieved by establishing a polypeptide capable of transferring α-glucosyl from a saccharide having an α-1,4-glycosyl bond as a binding manner at the non-reducing end and showing a degree of glucose polymerization of 2 or above to thereby synthesizing a saccharide having an α-1,6-glucosyl binding manner as a binding manner at the non-reducing end and an α-1,4-glycosyl bond as a binding manner other than at the non-reducing end and showing a degree of glucose polymerization of 3 or above without substantially elevating the reducing intensity; a DNA encoding this polypeptide; a replicable recombinant DNA containing the DNA encoding the above polypeptide and an autonomously replicable vector; a transformant constructed by transferring this recombinant DNA into an appropriate host; a process for producing the polypeptide; and use thereof.</p> |
