METHOD AND COMPOSITION FOR DETERMINING THE IMMUNOLOGICAL ACTIVITY OF CYTOKINE INDUCING SUBSTANCES AND THE IMMUNOLOGICAL RESPONSE TO THE THERAPY USING A CYTOKINE INDUCING SUBSTANCE

摘要

The immunological activity of certain bioactive substances is determined by treating a human peripheral blood leukocyte (PBL) culture or a Bulb/C mice resident peritoneal cells (RPC), such as abdominal membrane macrophage suspension, with a solution of the substance to be tested in order to induce production of cytokines which then are determined according to standard identification methods amplification of the results is achieved by admixing - to the solution to be tested - a non-steroidal anti-inflammatory drug, preferably a substance selected from the group of selenoorganic compounds such as Ebselen (R) of Nattermann (Rhône-Poulenc), indo-methacin and the p-chlorophenyl-amide of 3-methyl-5-benzoyl-amino-isothiazole-4-carboxylic acid.