SUGARCANE BACILLIFORM VIRAL (SCBV) ENHANCER AND ITS USE IN PLANT FUNCTIONAL GENOMICS

摘要

Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

主权项

1. A method of evaluating an expression profile of a sugarcane bacilliform virus (SCBV) construct, the method comprising:
operably linking (i) one or more SCBV enhancer elements having at least 90% sequence identity to position 337 to position 618 of SEQ ID NO: 1, (ii) a heterologous plant promoter sequence comprising an RNA polymerase binding site and a mRNA initiation site, and (iii) a heterologous expression sequence, thereby forming a SCBV construct; transforming a plant tissue with the SCBV construct; and evaluating the expression level of the expression sequence in the plant tissue.