| 摘要 |
PURPOSE:To form a nucleic acid fragment at high concn., by elongating the nucleic acid fragment in a reaction liquid containing no dideoxynucleotide and subsequently stopping the elongation of the nucleic acid fragment in the reaction liquid containing dideoxynucleotide. CONSTITUTION:In a first stage, all of nucleic acid fragments continue elongation because no dideoxynucleotide is contained and, in a second state, the elongation is stopped at the point of time when dideoxynucleotide was taken in the nucleic acid fragments having continued elongation. By enhancing the ratio of the quantity of dideoxynucleotide to deoxynucleotide in a reaction liquid in the second stage as compared with that of a conventional method, the elongation reaction of almost all of nucleic acid fragments can be stopped during the second stage. As a result, a short nucleic acid fragment or a long nucleic acid fragment no coming to an object to be detected is not contained or is contained at low concn. and a specimen containing a nucleic acid fragment having a length coming to an object to be detected is obtained.
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