SMART (SUBSURFACE MICROBIAL ACTIVITY IN REAL TIME) TECHNOLOGY FOR REAL-TIME MONITORING OF SUBSURFACE MICROBIAL METABOLISM

摘要

A sensor that measures microbial activity as a surrogate value for the biologically active content of soil, aquatic sediments, or groundwater. An anode, such as a graphite anode that can support a biofilm, is connected by way of a resistor to a cathode. The anode is in contact with either soil, sediment, or immersed in the groundwater of a subsurface monitoring well. The biofilm generates electrons as a consequence of chemical interactions with materials such as acetate dissolved in the soil or sediment waters or groundwater. The cathode is located in soil or water adjacent to the ground, which can be aerobic, so that a reaction that consumes electrons occurs at the cathode. The current flowing through the resistor is a measure of the biological activity at the anode, which correlates with the flux of fuel such as acetate to the anode.

主权项

1. A microbial activity sensor, comprising:
an anode electrode configured to support a biofilm on a surface thereof, and configured to be imbedded in the ground, in sediment, or immersed in groundwater, said anode electrode having an anode electrode terminal; a cathode electrode in electrical contact with the ground, sediment, or groundwater, said cathode electrode having a cathode electrode terminal; an electrical resistance connected between said anode electrode terminal and said cathode electrode terminal; and an electrical current monitor in electrical communication with said electrical resistance, said electrical current monitor configured to measure an electrical current passing through said electrical resistance, said electrical current monitor configured to record said a value representing measured electrical current at selected times, said electrical current monitor configured to report said recorded values representing measured electrical current and said selected times in response to an interrogation command.