Method of spatial high resolution imaging of a structure of a sample, the structure comprising a luminophore

摘要

For spatial high resolution imaging of a structure of a sample, the structure comprising a luminophore, the sample, in a measurement area, is subjected to an intensity distribution of luminescence inhibiting light comprising a local minimum. Then, the sample, in the measurement area, is subjected to luminescence excitation light which excites the luminophore out of an electronic ground state into a luminescent state, and luminescence light emitted out of the measurement area is registered. This registered luminescence light is assigned to the position of the local minimum within the sample. The luminescence inhibiting light disturbs the electronic ground state of the luminophore such that the luminophore, in the disturbed electronic ground state, has an absorption cross-section for the luminescence excitation light which is reduced by at least 50% as compared to the undisturbed electronic ground state.

主权项

1. A method of spatial high resolution imaging of a structure of a sample, the structure comprising a luminophore having an electronic ground state and a luminescent state into which the luminophore, out of its electronic ground state, is excitable by luminescence excitation light, the method comprising
in the measurement area, subjecting the sample to an intensity distribution of luminescence inhibiting light comprising a zero point, wherein the luminescence inhibiting light is configured to disturb the electronic ground state of the luminophore to such an extent that the luminophore, in its disturbed electronic ground state, has an absorption cross-section for the luminescence excitation light which is reduced by at least 50% as compared to an absorption cross-section for the luminescence excitation light of the luminophore in its undisturbed electronic ground state; in the measurement area, subjecting the sample to the luminescence excitation light exciting the luminophore out of its undisturbed electronic ground state into its luminescent state; registering luminescence light emitted out of the measurement area; and assigning the registered luminescence light to a position of the zero point within the sample.