BACTERIA AND METHOD FOR SYNTHESIZING FATTY ACIDS

摘要

There is provided a recombinant bacterium comprising at least one overexpressed acyl-ACP thioesterase gene, and wherein at least one gene from the tricarboxylic acid cycle or glycolysis or both is inactivated. There is also provided a method for producing fatty acids, said method comprising culturing bacteria comprising at least one overexpressed acyl-ACP thioesterase gene in a growth medium in a container having walls; allowing said bacteria to secrete fatty acids; and collecting said fatty acids. Acid supplementation is also shown to increase productivity.

主权项

1. A recombinant microorganism comprising at least one overexpressed acyl-ACP thioesterase, and wherein i) at least one protein from the tricarboxylic acid cycle is reduced, or ii) at least one protein from glycolysis is reduced, or both i) and ii) are reduced, wherein said at least one protein from glycolysis is selected from glucokinase, phosphoglucose isomerase, phosphofructokinase, aldolase, triose phosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, phophoglycerate kinase, phophoglycerate mutate, enolase, pyruvate kinase, and glucose phophotransferase.