| 发明名称 | Compositions and methods for increased expression in sugar cane | ||
| 摘要 | A sugar cane plant cell comprising a recombinant nucleic acid molecule is provided, the recombinant nucleic acid molecule comprising a nucleotide sequence of interest, a promoter and the nucleotide sequence of SEQ ID NO:1, wherein the promoter is downstream of and in operative association with the nucleotide sequence of SEQ ID NO:1 and upstream of and in operative association with the nucleotide sequence of interest, and the nucleotide sequence of interest is expressed at a level at least about 6 times greater than the level of expression of said nucleotide sequence of interest in a control. Additionally, a method of increasing the expression of a nucleotide sequence of interest in a sugar cane plant cell using the recombinant nucleic acid molecule of the invention is provided. | ||
| 申请公布号 | US9441234(B2) | 申请公布日期 | 2016.09.13 |
| 申请号 | US201214364184 | 申请日期 | 2012.12.07 |
| 申请人 | SYNGENTA PARTICIPATIONS AG;QUEENSLAND UNIVERSITY OF TECHNOLOGY | 发明人 | Kinkema Mark D.;Miles Stacy |
| 分类号 | C12N15/82 | 主分类号 | C12N15/82 |
| 代理机构 | Myers Bigel & Sibley, P.A. | 代理人 | Myers Bigel & Sibley, P.A. |
| 主权项 | 1. A sugar cane plant cell comprising a recombinant nucleic acid molecule, the recombinant nucleic acid molecule comprising a nucleotide sequence of interest, a promoter and the nucleotide sequence of SEQ ID NO:1, wherein the promoter is downstream of and in operative association with the nucleotide sequence of SEQ ID NO:1 and upstream of and in operative association with the nucleotide sequence of interest, and the nucleotide sequence of interest is expressed at a level at least about 6 times greater than the level of expression of said nucleotide sequence of interest in a control, further wherein the promoter is a maize polyubiquitin-1 promoter and/or a maize PepC promoter. | ||
| 地址 | Basel CH | ||