FABRICATION AND USE OF A MICROFLUIDICS MULTITEMPERATURE FLEXIBLE REACTION DEVICE

摘要

Fabrication of a microfluidic multi-temperature reaction device (MMR) and the design and fabrication of the equipment to drive various molecular biological methods on the device are provided. The device can be applicable, for example, to nucleic acid (DNA, RNA, cDNA, etc) amplification, cell lysis, reverse transcription and other enzymatic temperature sensitive and also temperature cycling reactions.

主权项

1. A device for conducting a biological reaction comprising:
a cassette that is configured to receive a sample; and one or more temperature-controlling elements, wherein said one or more temperature-controlling elements is configured to provide at least one temperature zone to the sample; wherein the cassette comprises one or more channels that are configured to move the sample through said at least one temperature zone; and the device is portable or handheld.