Monoclonal antibody against human pancreatic amylase, process for preparing the same and process for determining human pancreatic amylase by using the same.

摘要

A monoclonal antibody against human pancreatic amylase having a specific reactivity against human pancreatic amylase but completely no or almost no reactivity against human salivary amylase, a process for preparing a monoclonal antibody against human pancreatic amylase, which comprises forming hybridomas by fusion between antibody-producing cells of mice immunized with human pancreatic amylase and myeloma cells of mice, and culturing the hybridomas to collect monoclonal antibody against human pancreatic amylase from culture medium, and a process for immunologically determining human pancreatic amylase by employing the monoclonal antibody with specificity against human pancreatic amylase. According to the monoclonal antibody and the process of the present invention, only human pancreatic amylase can be selectively determined in a precise and rapid way in the determination of amylase for diagnosis of pancreatic diseases.