| 摘要 |
Glutamic acid producing Brevibacterium lactofermentum KFCC 10662 (I), which is unable to resist 0.03U/ml penicillin or 50mg/ml or more of nonionic sufactant Tween 40, can not use glutamic acid as C-source or N-source and produce glutamic acid in the waste syrup or culture medium contg. 98 g/l or more biotin concn., is prepd. by treating the original strain Brevibacterium lactofermentum ATCC 13869 with UV or NTG. Prodn. of glutamic acid comprises culturing (I) in a medium contg. waste syrup, glucose, starch hydrolyte, etc. and obtaining glutamic acid from the culture broth.
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