METHOD FOR PRODUCING SAMPLE AND METHOD FOR ANALYZING TARGET

摘要

The present invention is intended to provide a novel sensor for target analysis and a target analysis method using the same.;The sensor for target analysis according to the present invention includes a single-stranded nucleic acid molecule. The single-stranded nucleic acid molecule includes a first catalytic nucleic acid region (D1), a second catalytic nucleic acid region (D2), and a binding nucleic acid region (Ap) that binds to a target. The single-stranded nucleic acid molecule includes the first catalytic nucleic acid region (D1) at one end of the binding nucleic acid region (Ap) and the second catalytic nucleic acid region (D2) at the other end of the binding nucleic acid (Ap). In the absence of a target, the catalytic function by the first catalytic nucleic acid region (D1) and the second catalytic nucleic acid region (D2) is inhibited. In the presence of a target, the catalytic function is generated owing to formation of a G-quartet of the first catalytic nucleic acid region (D1) and the second catalytic nucleic acid region (D2) due to the contact between the target and the binding nucleic acid region (Ap).

主权项

1. A method for producing a sample, comprising:
bringing a specimen into contact with a cationic polymer in an aqueous mixture containing the specimen and the cationic polymer; recovering a liquid fraction containing a target in the specimen from the aqueous mixture by solid-liquid separation; and recovering a sample containing the target from the liquid fraction by column chromatography using an aqueous solvent, wherein the sample is a sample to be subjected to a method for analyzing a target using a catalytic nucleic acid molecule that generates a catalytic function.