SINGLE-STEP DNA PREPARATION FOR POLYMERASE CHAIN REACTION USING MAGNETIC CHITOSAN MICROPARTICLES
摘要
The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.
申请公布号
WO2016187563(A1)
申请公布日期
2016.11.24
申请号
WO2016US33571
申请日期
2016.05.20
申请人
CANON U.S. LIFE SCIENCES, INC.;UNIVERSITY OF MARYLAND, COLLEGE PARK
发明人
WHITE, Ian M.;RAGHAVAN, Srinivasa;PANDIT, Kunal R.;NANAYAKKARA, Imaly;CAO, Weidong