ACTIVATION OF MACROPHAGE TUMORICIDAL ACTIVITY BY GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR
摘要
Macrophages and precursor monocytes are activated to exhibit tumoricidal activity by stimulation solely with granulocyte-macrophage colony stimulating factor. A patient suffering from tumors can be treated by direct administration of therapeutically effective quantities of activated granulocyte-macrophage colony stimulating factor. Homogeneous granulocyte-macrophage colony stimulating factor for use in activating macrophages and monocyte precursors is prepared by recombinant DNA techniques. The gene coding for granulocyte-macrophage colony stimulating factor is isolated and then recombinant protein product expressed in an appropriate expression system. The granulocyte-macrophage colony stimulating factor recovered from the expression system is purified to homogeneity by reverse phase high-performance liquid chromatography.
申请公布号
CA1341142(C)
申请公布日期
2000.11.21
申请号
CA19860516034
申请日期
1986.08.15
申请人
IMMUNEX CORPORATION
发明人
COSMAN, DAVID J.;ANDERSON, DIRK M.;LARSEN, ALF D.;GRABSTEIN, KENNETH H.;PRICE, VIRGINIA L.;CONLON, PAUL J., III;CERRETTI, DOUGLAS P.;CANTRELL, MICHAEL A.