| 摘要 |
<p>PROBLEM TO BE SOLVED: To accurately classify and measure erythroblasts by dissolving red blood cell, mixing a fluorescent coloring matter for generating the difference in fluorescent intensity between leukocytes and erythroblasts for dyeing and performing measurement using a flow cytometer. SOLUTION: A blood sample and a hemolyzing agent are mixed, thus hemolyzing a red blood cell that becomes an obstacle when measuring leukocytes and erythroblasts. A fluorescent coloring matter is used for generating a difference in fluorescent intensity between the leukocytes and erythroblasts. The leukocyte cell is strongly dyed and emits strong fluorescence when being measured by a flow cytometer. On the other hand, the erythroblast is weakly dyed and emits a weak fluorescence. At least one scattered beam and at least one fluorescence are measured by the flow cytometer. Then, erythroblasts are classified and counted by using the intensity difference between the measured scattered beam and the fluorescence. For example, when a scattergram is drawn with a forward low-angle scattered beam at X axis and fluorescence at Y axis, each cell forms a group and is distributed. By analyzing the group with a proper analysis software, the number and the ratio of erythroblasts are calculated.</p> |
