PHASE CONTRAST MICROSCOPE

摘要

A phase contrast microscope includes a light source, an illumination optical system configured to irradiate an observation object with the light from the light source, and a magnifying optical system configured to magnify and project the observation object, the illumination optical system includes a ring slit, the magnifying optical system includes an objective provided with a phase film, the ring slit is disposed at a pupil position of the illumination optical system, the phase film is disposed at a position conjugate to the pupil position of the illumination optical system, and the following conditional expression (1) is satisfied:;r/I≦0.25  (1)wherer is a central part radius of the phase film, andI is a radius of a pupil of the objective.

主权项

1. A phase contrast microscope comprising:
a light source; an illumination optical system configured to irradiate an observation object with light from the light source; and a magnifying optical system configured to magnify and project the observation object, wherein the illumination optical system includes a ring slit, the magnifying optical system includes an objective provided with a phase film, the ring slit is disposed at a pupil position of the illumination optical system, the phase film is disposed at a position conjugate to the pupil position of the illumination optical system, and following conditional expression (1) is satisfied:
r/I≦0.25  (1) where r is a central part radius of the phase film, and I is a radius of a pupil of the objective.