| 主权项 |
1. A method for selecting a treatment for a subject having an inflammatory condition, the method comprising:
providing a plurality of biosensor complexes, each biosensor complex comprising a toll-like receptor (TLR) extracellular domain conjugated to a water-solubilized quantum dot, wherein at least one TLR extracellular domain is different from the other TLR extracellular domains of the biosensor complexes and at least one TLR extracellular domain is a TLR4 extracellular domain, wherein each biosensor complex further comprises a cognate ligand bound to the TLR extracellular domain, and wherein at least one cognate ligand is fluorescently labeled; providing a sample comprising a biological fluid or tissue from the subject; contacting the sample to the plurality of biosensor complexes; detecting a change in fluorescence to detect a change in binding of at least one cognate ligand to its TLR extracellular domain, wherein a change in binding indicates the presence of a ligand for the TLR extracellular domain in the sample; identifying which biosensor complex or complexes bind ligands present in the sample; and selecting a treatment for the subject based on the identity of the TLR extracellular domain bound by the ligand in the sample. |
