Method for detecting and quantifying wheat endogenous gene

摘要

Provided is a method of detecting or quantifying a wheat species-specific DNA in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having a partial sequence of a nucleotide sequence identified as SEQ ID NO: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule.

主权项

1. A kit for detecting or quantifying a wheat DNA sequence in a test sample by polymerase chain reaction, said kit comprising a primer pair and a circular DNA, wherein:
said primer pair comprises a first primer and a second primer, said circular DNA comprises a non-natural vector inserted with a nucleotide sequence capable of being amplified by polymerase chain reaction using said primer pair, said primer pair is capable of amplifying a partial sequence of the nucleotide sequence of SEQ ID NO: 1, (i) the first primer comprises the nucleotide sequence of SEQ ID NO: 4, and the second primer comprises the nucleotide sequence of SEQ ID NO: 5; or (ii) the first primer comprises the nucleotide sequence of SEQ ID NO: 4, and the second primer comprises the nucleotide sequence of SEQ ID NO: 6, the circular DNA further comprises at least one DNA consisting of a sequence specific to a strain of genetically modified wheat, and the sequence specific to a strain of genetically modified wheat comprises an exogenous DNA sequence inserted in the genome of the genetically modified wheat.