| 摘要 |
With respect to a sequencer pretreatment device and a method thereof, an object is to provide reliable pretreatment for analyzing a large-scale base sequence. Using is a sequencer pretreatment device including a suction and discharge mechanism, a nozzle head having a nozzle for detachably mounting a dispensing tip, a container group for accommodating various liquids including magnetic particle suspension, a moving mechanism for causing the nozzle relative movement with respect to the container group, and a magnetic unit capable of exerting a magnetic field to the mounted dispensing tip, the method is configured to include an extraction step of mixing a sample, extraction reagent solution, and magnetic particle suspension in the container group and extracting nucleic acid, a fragmentation producing step of fragmentating the nucleic acid by mixing with fragmentation solution accommodated in the container group and producing, from the fragmentated nucleic acid, a fragment of a base sequence having the number of bases corresponding to a sequencer using magnetic particle suspension using the sequencer pretreatment device, and an amplification pretreatment step of dispensing a predetermined volume of solution containing the produced fragment into the reaction vessel using the sequencer pretreatment device. |
