发明名称 |
METHODS OF PURIFYING ADENO-ASSOCIATED VIRUS (AAV) AND/OR RECOMBINANT ADENO-ASSOCIATED VIRUS (rAAV) AND GRADIENTS AND FLOW-THROUGH BUFFERS THEREFORE |
摘要 |
A method of purifying a viral vector is provided. The method includes loading a nonionic density gradient into a continuous flow centrifuge rotor; loading a material including the viral vector into the continuous flow centrifuge rotor; rotating the continuous flow centrifuge rotor in a manner sufficient to separate the viral vector and a predictable contaminate from the material in the nonionic density gradient; and unloading the separated viral vector and the predicable contaminant from the continuous flow centrifuge rotor. |
申请公布号 |
US2016201086(A1) |
申请公布日期 |
2016.07.14 |
申请号 |
US201614991510 |
申请日期 |
2016.01.08 |
申请人 |
ALFA WASSERMANN, INC. |
发明人 |
MERIÑO Sandra Patricia;CHEN Haifeng |
分类号 |
C12N15/86 |
主分类号 |
C12N15/86 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method of purifying a viral vector, comprising:
loading a nonionic density gradient into a continuous flow centrifuge rotor; loading a material including the viral vector into the continuous flow centrifuge rotor; rotating the continuous flow centrifuge rotor in a manner sufficient to separate the viral vector and a predictable contaminate from the material in the nonionic density gradient; and unloading the separated viral vector and the predicable contaminant from the continuous flow centrifuge rotor. |
地址 |
WEST CALDWELL NJ US |