ASSAYS FOR MEASURING BINDING KINETICS AND BINDING CAPACITY OF ACCEPTORS FOR LIPOPHILIC OR AMPHILPHILIC MOLECULES

摘要

Aspects of the invention relate to methods for measuring the binding constant of a lipophilic or amphiphilic molecule acceptor for a lipophilic or amphiphilic molecule. Methods involve rapid, cell-free competition assays including a labeled lipophilic or amphiphilic molecule and nanoparticle.

主权项

1. A method for measuring the equilibrium constant of an acceptor for a molecule, comprising:
(a) providing a lipophilic or amphiphilic molecule, wherein the molecule can provide a detectable signal; (b) providing a structure, the structure comprising a nanostructure core and a lipid layer surrounding and attached to the nanostructure core, wherein the structure quenches the signal of the molecule when the structure and the molecule are proximate; (c) providing an acceptor; (d) allowing the acceptor to compete with the structure for binding with the molecule; and (e) measuring the signal, wherein the level of the signal correlates with the equilibrium constant of the acceptor for the molecule.