发明名称 DETECTION METHOD OF TLR STIMULATING SUBSTANCE
摘要 PROBLEM TO BE SOLVED: To provide methods of sharply and rapidly detecting Listeria bacteria included in samples, such as environmental water, a flagellin derived from Salmonella bacteria, a lipopeptide derived from Mycoplasma bacteria, or a lipopolysaccharide derived from pathogenic bacteria.SOLUTION: By using a method comprising culturing under the presence of a sample a macrophage cell in which an expression cassette having a promoter sequence of IL-8 gene and a reporter gene sequence operably linked to the downstream of the promoter sequence is introduced, Listeria bacteria in the sample, a flagellin derived from Salmonella bacteria, a lipopeptide derived from Mycoplasma bacteria, or a lipopolysaccharide derived from pathogenic bacteria can be detected simply and highly sensitively.SELECTED DRAWING: None
申请公布号 JP2016208851(A) 申请公布日期 2016.12.15
申请号 JP20150092504 申请日期 2015.04.30
申请人 TOHOKU UNIV 发明人 KIMURA YUTAKA;AIBA SETSUYA
分类号 C12Q1/02;G01N33/569 主分类号 C12Q1/02
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