Methods for biosynthesizing 1,3 butadiene

摘要

This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.

主权项

1. A method for biosynthesizing butadiene, comprising
culturing a recombinant microbial host in a culture medium in the presence of a fermentable carbon source; enzymatically forming, in the recombinant microbial host, a first terminal vinyl group in 2-butanol using a polypeptide with the activity of a desaturase or a polypeptide with the activity of a monooxygenase to produce 3-buten-2-ol, wherein said desaturase is a cytochrome P450 or said monooxygenase is a gene product of mdpJ; and enzymatically forming, in the recombinant microbial host, a second terminal vinyl group in 3-buten-2-ol using a polypeptide with the activity of a dehydratase classified under EC 4.2.1- which is capable of accepting 3-buten-2-ol to biosynthesize butadiene by dehydration.