发明名称 |
Construction of novel strains containing minimizing genome by tn5-coupled cre/loxp excision system |
摘要 |
Disclosed is a method for developing novel strains deleted specific chromosome sites, using transposon and Cre/loxP site-specific recombination by Cre expression vector, wherein the transposon comprises a selectable marker and loxP site. The method comprises the steps of: (1) preparing a transposon comprising a selectable marker and loxP site; (2) inserting the transposon into an optional position of microbial chromosome, and determining the inserted site; (3) integrating two transposons comprising a different selectable marker to one chromosome; (4) deleting a chromosomal site between the two lox sites by introducing a Cre expression vector into the chromosome of step (3); and (5) repeating steps (3 and 4) for the mutant deleted a part of chromosome, to shorten the chromosome of mutant gradually.
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申请公布号 |
US2009104682(A1) |
申请公布日期 |
2009.04.23 |
申请号 |
US20020505328 |
申请日期 |
2002.10.31 |
申请人 |
KIM SUN-CHANG;YU BYUNG-JO |
发明人 |
KIM SUN-CHANG;YU BYUNG-JO |
分类号 |
C12N7/01;C12N15/09;C12N1/21;C12N15/63;C12N15/90 |
主分类号 |
C12N7/01 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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