TWO-DIMENSIONAL LIQUID CHROMATOGRAPHIC ANALYZER AND ANALYTICAL METHOD

摘要

A two-dimensional liquid chromatographic analyzer, in which a temperature control part changes a temperature in a single-step manner and switches the temperature at a high speed. Also, the temperature control part has a holder around a separation column so that the temperature of the separation column can be changed to a preset temperature. A first separation column thereof changes elution time(s) of the objective component(s) by temperature modulation and the mobile phase of the first separation column is an aqueous mobile phase having a constant composition.

主权项

1. A two-dimensional liquid chromatographic analyzer comprising:
a liquid feed part configured to feed a mobile phase; an injection part configured to inject a sample; a first separation column configured to separate and fractionate the sample containing a plurality of components; a second separation column configured to separate the fractionated components; a first temperature control part configured to control temperature of the first separation column; a second temperature control part configured to control temperature of the second separation column; a detection part configured to detect the separated components, wherein the first temperature control part comprises a holder made of metal and configured to hold the first separation column so as to transmit a temperature change of the first temperature control part to the first separation column.