| 发明名称 | Nucleic acid extraction device, and nucleic acid extraction method, nucleic acid extraction kit, and nucleic acid extraction apparatus, each using the same | ||
| 摘要 | A nucleic acid extraction device includes a tube that is internally provided with, in the following order, a first plug composed of a first oil, a second plug composed of a first washing liquid, which is phase-separated from an oil and is used for washing a nucleic acid-binding solid-phase carrier having nucleic acids bound thereto, a third plug composed of a second oil, a fourth plug composed of a reverse transcription reaction solution, which is phase-separated from an oil and is used for performing a reverse transcription reaction, a fifth plug composed of a third oil, a sixth plug composed of an eluent, which is phase-separated from an oil and is used for eluting the nucleic acids from the nucleic acid-binding solid-phase carrier having nucleic acids bound thereto, and a seventh plug composed of a fourth oil. | ||
| 申请公布号 | US9347056(B2) | 申请公布日期 | 2016.05.24 |
| 申请号 | US201314062228 | 申请日期 | 2013.10.24 |
| 申请人 | Seiko Epson Corporation | 发明人 | Saito Yuji;Takagi Fumio |
| 分类号 | C12P19/34;C12N15/10;B01D15/00;G01N30/60;B01L3/00;B01D15/18;B01D15/22;G01N30/00 | 主分类号 | C12P19/34 |
| 代理机构 | Harness, Dickey & Pierce, P.L.C. | 代理人 | Harness, Dickey & Pierce, P.L.C. |
| 主权项 | 1. A nucleic acid extraction device, comprising a tube that is internally provided with, in the following order from a first end of the tube to a second end of the tube: a first plug composed of a first oil, a second plug composed of a first washing liquid, which is phase-separated from an oil and is configured to wash a nucleic acid-binding solid-phase carrier having nucleic acids bound thereto, a third plug composed of a second oil, a fourth plug composed of a reverse transcription reaction solution, which is phase-separated from an oil and is configured to perform a reverse transcription reaction, a fifth plug composed of a third oil, a sixth plug composed of an eluent, which is phase-separated from an oil and is configured to elute the nucleic acids from the nucleic acid-binding solid-phase carrier having nucleic acids bound thereto, and a seventh plug composed of a fourth oil, wherein the device is configured such that the nucleic acid-binding carrier can move from the first plug, through the second, third, fourth, and fifth plugs, and to the sixth plug. | ||
| 地址 | JP | ||