Engineered tissues for in vitro research uses, arrays thereof, and methods of making the same

摘要

Disclosed are living, three-dimensional tissue constructs for in vitro scientific and medical research, arrays thereof, and methods of making said tissues and arrays. WO 2013/040078 PCT/US2012/054923 Fig. 13 D E t> F Figure 13: Analysis of multi-layered blood vessel wall segments printed on multi-well cell culture inserts under static and flow conditions. Hematoxylin and Eosin staining of formalin-fixed paraffin-embedded tissues reveals well formed tissue constructs under both static (A) and flow/shear stress (D) culture conditions. Static culture of blood vessel constructs was sufficient to maintain proper cell arrangement which was characterized by a layer of CD3 1 -positive EC at the laminar surface (C) and an a-SMA positive SCM-rich media. Following exposure to 5 mL/min flow in a flow cell chamber, the CD3 1 positive EC layer appeared thicker (E) and the a-SMA-positive SMC-rich media appears to also be thicker and more well-organized, suggestive of a positive response to the biomechanical stimuli associated with shear stress and fluid flow.