| 摘要 |
The invention relates to membrane preparations for use in an increased sensitivity ABCG2 protein activity assay, said preparations comprising liposomes and one or more bile acid compounds wherein the bile acid compounds reduce the basal ATPase activity in said liposomes. Said membrane preparations can be cell membrane preparations or a membrane protein preparations. The invention also relates to methods for obtaining said membrane preparations, either cell membrane preparations or, preferably, membrane protein preparations comprising a reconstituted, recombinant ABCG2 protein. The invention also relates to kits comprising said membrane preparations as well as uses said kits, membrane preparations or bile acid compounds in high sensitivity ABCG2 protein activity assays.. |
