THERMOSTABLE GLYCOSIDE HYDROLASE

摘要

A thermostable glycoside hydrolase having a glycoside hydrolase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0; (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0; (D) a polypeptide in which are linked, either directly or via a linker, a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the region composed of amino acids from positions 51 to 485 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA at least under conditions of 65° C. and pH 4.0, and a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the region composed of amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0; or (E) a polypeptide in which are linked, either directly or via a linker, a polypeptide including an amino acid sequence having 80% or greater sequence identity with the region composed of amino acids from position 51 to position 485 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of PSA at least under conditions of 65° C. and pH 4.0, and a polypeptide including an amino acid sequence having 80% or greater sequence identity with the region composed of amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0.

主权项

1. A thermostable glycoside hydrolase, having a glycoside hydrolase catalytic domain comprising:
(A) a polypeptide comprising an amino acid sequence represented by SEQ ID NO: 1, (B) a polypeptide comprising an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel and hydrolysis activity against a substrate of carboxymethyl cellulose at least under conditions of 65° C. and pH 4.0, (C) a polypeptide comprising an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel and hydrolysis activity against a substrate of carboxymethyl cellulose at least under conditions of 65° C. and pH 4.0, (D) a polypeptide in which are linked, either directly or via a linker, a polypeptide comprising an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in a region comprising amino acids from positions 51 to 485 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 65° C. and pH 4.0, and a polypeptide comprising an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in a region comprising amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of carboxymethyl cellulose at least under conditions of 65° C. and pH 4.0, or (E) a polypeptide in which are linked, either directly or via a linker, a polypeptide comprising an amino acid sequence having 80% or greater sequence identity with a region comprising amino acids from position 51 to position 485 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 65° C. and pH 4.0, and a polypeptide comprising an amino acid sequence having 80% or greater sequence identity with a region comprising amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of carboxymethyl cellulose at least under conditions of 65° C. and pH 4.0.