Vaccine against Newcastle fowl disease, method for preparing and use thereof

摘要

The vaccine against Newcastle fowl disease incorporates a virus-containing extra-embryonic liquid of a lentogenic strain Paramyxovirus a-1 "Bor-74 VGNKI" deposited at the All-Union State Research and Control Institute for Veterinary Preparations of the USSR Ministry of Agriculture and has a biological acitivity of from 108.0 to 109.75 EID50/ml. The method for preparing this vaccine comprises infectioning chicken embryos with the strain Paramyxovirus a-1 "Bor-74 VGNKI", preparation of a matrass breeding culture of the virus at the temperature of 37 DEG -37.5 DEG C. for 72-80 hours followed by isolation of the virus-containing extra-embryonic liquid and manufacture of the vaccine. The vaccine is employed for prophylaxis of Newcastle fowl disease by way of administration thereof once or twice onto mucous membrane of the fowl eye or nose in a dose of from 0.05 to 1.0 ml at the dilution of 1:100 or by way of enteral administration with potable water to chicks of an age of up to 25 days in a single dose of from 7.5 to 10 ml at the dilution of 1:1,000, followed by revaccination of the elder fowl, or by administration as an aerosol to the fowl of an age under 25 days in a dose of 300-400 EID50, followed by revaccination of the elder fowl in a dose of from 800 to 1,000 EID50.