SHUTTLE PROMOTER DNA SEQUENCE IN E.COLI AND YEAST

摘要

The new DNA(YEP1) having promotor activity in E.coli and yeast and its purification method are presented. Thus, DNA extracted from yeast is treated with restriction enzyme AluI and HaeIII, and DNA with 500-2500 base pairs is obtained by electrophoresis in 1% agarose gel. The obtained DNA is treated with restriction enzyme BamHI and ligated into pk15 vector using T4 DNA ligase. E.coli LE392 transformed with above vector is cultured and plasmid DNA is extracted from E.coliLE392. Yeast transformed with extracted plasmid DNA is treated with restriction enzyme BamHI.