QUANTITATION BY SERIAL COMBINATORIAL DILUTION

摘要

The invention provides a method for the quantification of a biomolecule in a complex mixture of biomolecules which comprises a fractionation of the mixtu re of biomolecules providing at least two fractions with at least one distinct component each. These fractions are then subjected to serial combinatorial dilution. Subsequently, the biomolecule is detected and identified in the fractions by a method providin g a sensitivity threshold and identify information. The quantity of the biomolecule is determined by summarizing the number of identifications of the biomolecule in each fractio n on each dilution level in consideration of the respective dilution factor. For purpo se of normalization this sum may be divided by the total number of identifications of all biomolecules in all fractions on all dilution levels.