Detection of mycobacteria by multiplex nucleic acid amplification
摘要
Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of Mycobacterium tuberculosis (M.tb) and the 16S ribosomal gene of Mycobacterium tuberculosis, useful for simultaneously detecting and/or identifying species of the M. tuberculosis complex and other clinically relevant Mycobacterium species. Multiplex Strand Displacement Amplification (SDA) is used in a single amplification reaction which is capable of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of Mycobacteria. Also disclosed are methods for adapter-mediated multiplex amplification of multiple target sequences and a single internal control sequence for determination of sample efficacy or quantitation of the targets. In a preferred embodiment, an internal control sequence is included in the amplification reaction and coamplified with the IS6110 and 16S target sequences as an indication of sample amplification activity or to quantitate the initial amount of target sequences in the sample.
申请公布号
SG44897(A1)
申请公布日期
1997.12.19
申请号
SG19960009112
申请日期
1994.08.16
申请人
BECKTON, DICKINSON & COMPANY
发明人
JURGENSEN STEWART RUSSEL;NADEAU, JAMES, G.;NYCZ, COLLEEN, M.;SCHRAM, JAMES, L.;SHANK DARYL LEE;SPEARS PATRICIA ANNE;WALKER GEORGE TERRANCE