Yeast secretion vectors.

摘要

A vector includes a DNA sequence encoding a secretory signal sequence substantially identical to the secretory signal-encoding sequence of a glucoamylase gene from Saccharomyces diastaticus or S. cerevisiae. Upstream from the signal-encoding sequence is a DNA sequence capable of promoting transcription in yeast, transcription of the signal-encoding sequence being under the control of the transcription-promoting sequence. A site for the insertion into the vector of a heterologous DNA sequence is in reading frame with the signal-encoding sequence.