DESIGN, CONSTRUCTION AND EXPRESSION OF CHIMERIC PROTEINS FOR DEVELOPMENT OF VACCINES AND DIAGNOSTIC REAGENTS

摘要

The chimeric proteins, and the vaccine compring the chimeric protein are disclosed. The preparation comprises linking gag of HIV to env to form the chimeric gene, inserting the obtained chimeric gene into the DNA of baculovirus, infecting the resulted recommbinant virus with the insect cells or insect, culturing it and purifying the obtained chimeric protein. The gag chimeric protein of HIV according to the present invention retains both antigenic and immunogenic properties.