Method of colorimetry and reagent for use therein

摘要

Disclosed is a colorimetric method including: transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent; wherein the mediator is at least one selected from the group consisting of a copper complex, an iron complex, an osmium complex, and a ruthenium complex. Further disclosed are: 1) a reagent used for the colorimetric method according to claim 1, including: an oxidoreductase; a mediator; and a coloring reagent for producing color by reduction, wherein the mediator is at least one selected from the group consisting of a copper complex, an iron complex, an osmium complex, and a ruthenium complex; and 2) a test piece for colorimetric analysis which includes the reagent defined above.