Leukemic stem cell markers

摘要

The present invention relates to a method for identifying Leukemic stem cells (LSC), based on measurement of the presence of a specific set of markers. The invention further relates to a container for use in performing the method. Also provided is application of the method for predicting response to therapy and/or chance of relapse of Acute Myeloid Leukemia, and application of the method in the context of screening for compounds that specifically eradicate Leukemic cells and not benign cells.

主权项

1. Method for identifying Leukemic stem cells (LSC), the method comprising:
(a) providing a sample obtained from a subject having or suspected of having Acute Myeloid Leukemia or Myelodysplastic Syndrome; (b) measuring, for individual cells of the sample, expression of a set of at most 20 markers, the set comprising markers CD34, CD38, CD45, CD123, CD33, CLL-1, TIM-3, CD11b, and CD22; wherein an individual cell is identified as a Leukemic stem cell if the cell is CD34+, CD38−, CD45+, and at least one of CD123+, CD33+, CLL-1+, TIM-3+, CD11b+, and CD22+.