| 摘要 |
The invention relates to a method for producing pancreatic carboxy-peptidase B or an isoform or a mutein of carboxy-peptidase B, whereby: (a) a natural or unnatural precursor form of carboxy-peptidase B is expressed in a secretory manner in a microorganism; (b) the precursor form expressed in a secretory manner is purified, and; (c) the purified precursor form is converted into the active carboxy-peptidase B or into the isoform or the mutein thereof by means of an enzymatic treatment. The invention also relates to a nucleic acid construct, to its use in the aforementioned method, and to the production thereof, as well as to a host cell, the production thereof and its use in the method for producing pancreatic procarboxy-peptidase B or an isoform or a mutein thereof.
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