Methods and systems for detecting an analyte in a sample

摘要

The present disclosure provides methods for the detection of one or more analytes in a sample. Aspects of the methods include flowing a sample (e.g., a biological sample, such as blood) through a channel comprising an analyte specific capture domain stably associated with a surface thereof, wherein the analyte specific capture domain comprises particles displaying a specific binding member for an analyte; and imaging the analyte specific capture domain to detect whether the analyte is present in the sample. Also provided are systems, devices, and kits that may be used in practicing the subject methods. Methods and compositions as described herein find use in a variety of different applications, including diagnostic applications.

主权项

1. A method of detecting whether an analyte is present in a sample, the method comprising:
(a) contacting a sample with a fluorescent label that specifically binds to the analyte to produce a fluorescently labelled sample; (b) flowing the fluorescently labelled sample through a capillary channel of a cartridge device comprising a first analyte specific capture domain on an inner surface of the capillary channel at a known location, wherein the first analyte specific capture domain comprises:
(i) particles adhered to the surface of the capillary channel; and(ii) a binding member that specifically binds to fluorescently labeled analyte of the fluorescently labeled sample, wherein the binding member is contained in and/or on a surface of the particles and wherein the capillary channel has a defined height to particle ratio; and (c) imaging the first analyte specific capture domain comprising the adhered particles in the capillary channel to detect whether fluorescently labeled analyte is bound by the binding member of the particles and therefore present in the sample, wherein the imaging is performed without washing to remove unbound sample components prior to imaging.