| 摘要 |
<p>Human glutathione peroxidase (GPx) is of potential therapeutic use for a variety of disorders stemming from a variety of peroxides which are metabolic products. However, sources of human GPx are extremely limited. The present invention provides a method for synthesizing human GPx on a large scale by use of recombinant DNA techniques. In the present invention, a polynucleotide sequence coding for human GPx is provided. The polynucleotide sequence, or its modified forms, may be inserted into expression vectors allowing the synthesis of recombinant products which include human GPx, fragments of human GPx, analogs of human GPx, and analogs of fragments of human GPx. These recombinant polypeptide products have potential therapeutic use. In addition, they may be used to raise antibodies, both monoclonal and polyclonal, to human GPx. Antibodies to human GPx are useful for the diagnosis of deficiencies in human of GPx and of selenium. Also, analog of human GPx, active fragments of GPx, and monoclonal antibodies to human GPx are all useful for determining how the enzyme interacts with its various substrates, as well as how the enzyme may be targeted for hydrophilic and hydrophobic sites. This knowledge will be useful in designing analogs of human GPx for specific therapeutic uses.</p> |
