| 摘要 |
<p>A sample is separated and separated components thereof are successively fed to a part to be detected (2c). A laser beam of at least 600nm from a laser beam source (212) of an optical measuring part (210) is applied to the part to be detected (2c) through a dichroic mirror (214) and a lens (216), for making a fluorochrome bonded to the separated components absorb multiphotons, exciting the fluorochrome and making the same fluoresce. The optical measuring part (210) captures the fluorescence so that photomultipliers (220,224,228,230) detect fluorescence of not more than 510nm in wavelength, fluorescence longer than 510nm and not more than 560nm in wavelength, fluorescence longer than 560nm and not more than 580nm in wavelength and fluorescence longer than 580 in wavelength respectively. Thus, a capillary electrophoretic apparatus can detect fluorescence from a fluorochrome bonded to samples as a label without influence by Raman scattering or Rayleigh scattering. <IMAGE></p> |
