AN IMPROVED METHOD OF DNA SEQUENCING

摘要

An improved method of determining the nucleotide base sequence of DNA is disclosed. The method comprises labeling and protecting predetermined regions of a strand of nucleic acid to be sequenced to produce a blocked and labeled strand having blocked, labeled and unblocked regions. Subsequently, the blocked and labeled strand is treated with a cleavage reagent that preferentially cleaves the strand at a point in the unblocked region to produce a labeled fragment having a blocked region. The nucleotide sequence of at least a portion of the blocked region is then determined.