Media for culturing, preserving, and administering regenerative cells

摘要

A culture media, media supplement, or soluble matrix for cryopreservation or enhanced regenerative cell growth in culture and maintenance of multi-lineage differentiation potentiation. The inventive culture media, media supplement, or soluble matrix comprises a GAG composition comprising a sulfated GAG, such as chondroitin sulfate. A soluble matrix, a cell administration package or kit comprising the soluble matrix and a device for cell administration, and a method of use thereof, for administration of regenerative cells for treating a joint disease or other weakened or damaged tissue comprising the specified GAG compositions are further provided.

主权项

1. A method of culturing cells having multi-lineage differentiation potential, comprising culturing the cells for at least 24 hours in a cell culture media comprising a glycosaminoglycan (GAG) composition, wherein said GAG composition comprises hyaluronic acid, chondroitin sulfate and N-acetyl D-glucosamine in a relative weight ratio of 1:20:20, wherein said GAG composition is at a concentration of 5-10% (v/v), and wherein the cultured cells have an enhanced in vitro expansion rate and maintain multi-lineage differentiation potential compared to cells having a multi-lineage differentiation potential not cultured in the media.