Methods and Devices for Imaging Large Intact Tissue Samples

摘要

Methods and devices for conducting high-speed, high-resolution imaging of large intact tissue samples are provided. Aspects of the methods include placing a sample in an optically homogenous sample manipulation component, performing a calibration procedure to align a light sheet and a detection focal plane at a plurality of locations within the sample, and performing an imaging procedure on the sample to collect an image from each location. The collected images are reconstructed to form a three-dimensional image of the sample. Devices for carrying out the steps of the methods are also provided.

主权项

1. A microscope device comprising:
an illumination beam path that comprises a light source; a detection beam path that comprises a camera; an optically homogenous sample manipulation component that comprises a sample chamber having a volume; a controller; a processor; and a computer-readable medium comprising instructions that, when executed by the processor, cause the controller to:
execute a calibration procedure to acquire a plurality of alignment parameters for a sample placed in the sample chamber; andexecute an imaging procedure that utilizes the alignment parameters to generate a three dimensional image of the sample.