FREEZE-DRIED POWDER OF HIGH MOLECULAR WEIGHT SILK FIBROIN, PREPARATION METHOD THEREFOR AND USE THEREOF

摘要

The invention discloses a freeze-dried powder of high molecular weight silk fibroin, preparation process and use thereof. The freeze-dried powder is obtained from silk by degumming, dissolution, dialysis, centrifugation, high temperature and high pressure treatment and freeze-drying. A method of preparing silk fibroin microspheres with polyethylene glycol comprises mixing a silk fibroin solution of 1-30 wt % with a PEG solution of 10-60 w % and incubating the resulting solution. A controlled-release or sustained-release silk fibroin gel formulation includes a gel-state carrier and a drug dispersed/adsorbed therein, the carrier is a silk fibroin gel formed by blending with LMW-PEG. The freeze-dried powder will not change the native molecular weight and random coil structure of silk fibroin, and can be rapidly dissolved into a solution, and can be stored and transported for a long time, and this reduces waste of silk fibroin materials and promotes R & D and biomedical applications of silk fibroin biomaterials.

主权项

1. A preparation process of a freeze-dried powder of high molecular weight silk fibroin, comprising the steps of:
(1) boiling silk in an amount of a sodium carbonate solution for a period of time, and then soaking and washing with clear water to get degummed silk; (2) after air drying of the degummed silk, dissolving a suitable amount of the degummed silk in a lithium bromide solution; (3) after complete dissolution, dialyzing the degummed silk in a dialysis tube for two days; (4) after dialysis, centrifuging the silk fibroin solution, and taking the supernatant as a silk fibroin solution; and (5) after determining the concentration of the solution, mixing it with a phosphate buffer solution to prepare a silk fibroin solution, treating the solution with high temperature and high pressure, transferring a volume of the solution into a glass bottle and freezing overnight, and then freeze-drying for a period of time to obtain the freeze-dried powder of high molecular weight silk fibroin.