Production of insulin producing cells

摘要

The invention provides methods for differentiating stem cells along the pancreatic lineage as well as large scale culture methods. The present invention further provides pancreatic progenitor cells derived from stem cells to provide pancreatic cells to a subject.

主权项

1. A method to provide pancreatic cells to a subject in need thereof comprising:
a) contacting the stem cells with at least one member of the TGFβ family of cytokines and at least one member of the Wnt family of proteins, wherein the stem cells are induced pluripotent stem (iPS) cells, b) contacting the cells obtained from step a) with at least one member of the TGFβ family of cytokines, at least one member of the Wnt family of proteins, and an agent that inhibits sonic hedgehog activity; and c) contacting the cells obtained from step b) with a member of the Epidermal growth factor (EGF) family of proteins; and administering the cells so as to provide pancreatic cells in the subject.