| 摘要 |
Provided is a method whereby it becomes possible to carry out a pretreatment for gene testing in a POCT mode. A pretreatment method according to the present invention comprises: bringing a material to be treated, an extraction solution for extracting a nucleic acid contained in the material to be treated, silica particles and a filtering material into contact together to cause a composite material composed of the nucleic acid and the silica particles to be supported on the filtering material; and delivering the composite material-supported filtering material to a nucleic acid amplification step in which a nucleic acid amplification reaction solution is used. In the pretreatment method, a drying step and an elution step, which are normally carried out in advance of the nucleic acid amplification step, can be eliminated by keeping the particle diameters of the silica particles and the concentration of the silica particles in the nucleic acid amplification reaction solution in specified range, respectively. The specified range for the concentration of the silica particles is 0.0625 to 4 μg/μl, the specified range for the average particle diameter of the silica particles is 0.01 to 100 μm, and the surface area of the silica particles, which is determined from the average particle diameter, is 1 × 10^4 to 1 × 10^8 μm^2. |
