Test for predicting neutralization of asparaginase activity

摘要

Method of in vitro measurement of the presence of factors that are able to neutralize asparaginase activity in a sample of blood, plasma, serum or derived medium that may contain asparaginase neutralizing factors, obtained from a patient, comprising mixing of said sample with asparaginase, incubation of said mixture, then measurement of the residual asparaginase activity in the mixture and determination or quantification of the presence of said neutralizing factors. Method for predicting the efficacy of a treatment with asparaginase.

主权项

1. A method of treatment of an asparaginase-sensitive pathology in a patient, said methods comprising:
(a) obtaining a sample of blood, plasma, or serum from a patient; (b) incubating said sample with a known amount of asparaginase for a period of time sufficient to produce antibody-asparaginase immune complexes; (c) removing from the incubated sample any antibody-asparaginase immune complexes formed during incubation step (b) and recovering a sample free of antibody-asparaginase immune complexes; (d) incubating the sample obtained at step (c) with asparagine; (e) determining a residual asparaginase activity or the amount of asparaginase residual activity in the resultant mixture of step (d); and (f) treating said patient by means of the asparaginase and/or by means of another form of asparaginase.